Biochemistry of Metabolism: Cell Biology

Microtubules

Reading:

Textbook Reading: Alberts et al. 4th Edition Chapter 16. Specific pages given in the notes.

Some recent articles (optional reading): 
J. Lwe, H. Li, K. H. Downing & E. Nogales (2001) "Refined structure of ab-tubulin at 3.5 resolution," J. Mol. Biol. 313: 1045-1057.
S. I. Dutcher (2001) "The tubulin fraternity: alpha to eta," Curr. Opin. Cell Biol. 13: 49-54.
M. Moritz & D. A. Agard (2001) "g-Tubulin complexes and microtubule nucleation," Cur. Opin. Struct. Biol. 11: 174-181.
C. L. Rieder, S. Faruki & A. Khodjakov (2001) "The centrosome in vertebrates: more than a microtubule-organizing center," Trends in Cell Biol. 11: 413-419.
K. Kinoshita, B. Habermann & A. A. Hyman (2002) "XMAP215: a key component of the dynamic microtubule cytoskeleton," Trends in Cell Biol: 12: 267-273.
S. G. Addinall & B. Holland (2002) "The tubulin ancester, FtsZ, draughtsman, designer and driving force for bacterial cytokinesis," J. Mol. Biol. 318: 219-236.
M. Bornens (2002) "Centrosome composition and microtubule anchoring mechanisms," Current Opinion in Cell Biol. 14: 25-34.
D. Job, O. Valiron & B. Oakley (2003) "Microtubule nucleation," Current Opinion in Cell Biol. 15: 111-117.
JA. Dammermann, A. Desai & K. Oegema (2003) "The minus end in sight," Current Biol. 13: R614-R624.
J. Howard & A. A. Hyman (2003) "Dynamics and mechanics of the microtubule plus end," Nature 422: 753-758.
J. Beisson & M. Wright (2003) "Basal body/centriole assembly and continuity," Current Opinion in Cell Biol. 15: 96-104.
Y. Ou, M. Zhang & J. B. Rattner (2004) "The centrosome: the centriole-PCM coalition," Cell Motility & Cytoskeleton 57: 1-7.
G. C. Rogers, S. L. Rogers & D. J. Sharp (2005) "Spindle microtubules in flux," J. Cell Sci. 118: 1105-1116.
X. Xiang (2006) "A + TIP for a smooth trip," J. Cell Biol. 172: 651-654.
X. Wu, X. Xiang & J. A. Hammer III (2006) "Motor proteins at the microtubule plus-end," TRENDS in Cell Biol 16: 135-143.
C. Wiese & Y. Zheng (2006) "Microtubule nucleation: g-tubulin and beyond," J. Cell Sci. 119: 4143-4153.

Potential Test Questions:

1.a. Describe the structure and composition of an individual microtubule. What is the basic unit that adds or dissociates during lengthening or shortening of a microtubule? What nucleotides are associated with these units, and where are they located within the molecule? What is the fate of the nucleotides after polymerization? What is thought to function as GTPase activating protein (GAP)? 
b. Describe and explain the dynamic instability exhibited by some microtubules at their plus end.
c. Summarize the composition and role of the g-tubulin ring complex.

Copyright 1999-2006 by Joyce J. Diwan. All rights reserved.

Lecture Notes on
Microtubules

Interactive Quiz on    
    Microtubules       

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